Antioxidant effect of rosmarinic acid against renal ischemia reperfusion injury in rat; a histopathological study

Majid Tavafi, Hamid Reza Sameni, Ahmad Tamjidipour, Afshin Hasanvand, Hesam Ashoory

Abstract


Abstract

Introduction: Renal ischemia reperfusion (I/R) occurs during renal surgical procedure that leads to renal injuries and can progress to acute renal failure. Objectives: Due to central role of reactive oxygen species (ROS) in pathogenesis of renal I/R, rosmarinic acid (RA) an antioxidant agent were used against renal I/R injuries. Materials and Methods: Forty male mature Sprague Dawley rats (180–200 g) were divided into five groups (8 rats per group) as follows; group 1 control; group 2 I/R, without treatment; group 3 I/R + RA (50 mg/kg); group 4 I/R + RA (100 mg/kg) and group 5 I/R + RA (200 mg/kg). RA was given orally by gavage in groups 3, 4 and 5 one hours before surgery. I/R induced through ligation of renal pedicles bilaterally for 45 minutes and then 24-hour reperfusion. After 24 hours, blood were sampled, Left kidney fixed in formal saline solution and then tissue section (5 µ thickness) prepared and were stained with PAS method. Serum malondialdehyde (MDA), urea and creatinine were measured. Histological variables were assessed morphometrically and semiquantitativly. The data compared by SPSS 13 and Mann-Whitney U test at P<0.05. Results: Histological injuries (tubular necrosis, casts, diameter, tubular volume density, mean glomerular volume) and serum creatinine, urea, MDA were improved in RA treated groups in comparison with group 1 (P<0.05), but the treatment cannot be kept variables at the same level as that of control group. Conclusion: Histopathological injuries of renal I/R, were reduced by RA via inhibition of lipid peroxidation and finally led to amelioration of serum urea and creatinine.

 

Core tip

In an experimental study, histopathological injuries of renal ischemia reperfusion, were reduced by rosmarinic acid through inhibition of lipid peroxidation and finally lead to amelioration of serum urea and
creatinine.

 

Citation

Tavafi M, Sameni HR, Tamjidipour A, Hasanvand A, Ashoory H. Antioxidant effect of rosmarinic acid against renal ischemia reperfusion injury in rat; a histopathological study. Ann Res Antioxid. 2016;1(2):e24.


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